Research Interests

We welcome highly motivated and creative individuals to join the lab. If you are interested in any of the projects listed below please contact the appropriate person listed at the end of each project description. Expressions of interest with a curriculum vitae can be sent to Professor Adam Jaffe.

> Creating model systems for precision medicine

We are using an exciting and cutting edge technology to grow stem cell-derived human ‘mini organs’ (organoids) from the tissues of patients with CF.  These organoids are characterised by genome sequencing, expression profiling and sensitivity to known drugs.  By recording and analysing this information we can predict a patient’s response to pre-clinical and clinical drugs, allowing us to rapidly develop effective treatments tailored to individuals.
Contact: Dr Shafagh Waters

> MATRIX-CF: Modelling the Alimentary Tract using ex-vivo systems in Cystic Fibrosis study

The primary aim of this project is to culture an intestinal organoid model of CF and healthy control patients, and to then use these models to compare the responses of each to microbial communities.These can then be used to observe the complex interactions between host (genetic, immune and mucosal) factors, microbial communities and intestinal inflammation.  Secondary aims are to test the effects of drugs or interventions which have theoretical or potential anti-inflammatory effects on the intestinal model in CF.
Contact: Dr Shafagh Waters or Dr Keith Ooi

> RNA-targeted activation of CFTR

The overall goal of this project is to identify intrinsic molecules that disrupt production of the CFTR protein in the cell. We have identified one such molecule and our aim is to find out its prevalence in people with CF.
Contact: Dr Shafagh Waters

> PEARL-CF: Probiotics and early-life effects on intestinal bacteria and inflammation

This study focuses on the unique environment that exists in the gastrointestinal tract of children with cystic fibrosis, paying close attention to changes in gut microbiota and the development of intestinal inflammation. Work is concentrated on trying to understand the way the disease develops, its clinical impact and potential therapies.
Contact: Dr Keith Ooi

> DISH-CF: Dietary Intake Study in children with Cystic Fibrosis

This study evaluates the dietary intake of children with Cystic Fibrosis. Ideally, this will lead to the identification of optimal eating patterns to support health and wellbeing, and inform future global nutritional recommendations in Cystic Fibrosis.
Contact: Dr Keith Ooi

> EOS-CFRD: Early Origins Study of Cystic Fibrosis-related Diabetes study

Some children with Cystic Fibrosis eventually develop Cystic Fibrosis-related Diabetes, however very little is known about how diabetes develops over time. This study examines the glucose levels of young children to get a better understanding of diabetes in Cystic Fibrosis, and aims to facilitate its early detection.
Contact: Dr John Widger or Dr Bernadette Prentice

> INSPPIRE: International Study Group of Pediatric Pancreatitis

In association with the Early Origins Study of Cystic Fibrosis-related diabetes (above), we hope to better understand pancreatitis in children and to develop better tests and treatments.INSPPIRE is a group of 21 paediatric gastroenterology centres in the United States, Canada, Israel, and Australia.
Contact: Dr Keith Ooi

> Serum biomarkers of Cystic Fibrosis-related Diabetes

This project aims to detect biomarkers that can be used to identify individuals with CF at risk of developing complications such as cystic fibrosis-related diabetes. This would result in more accurate predictions and improve the care and long-term outcomes of CF patients.
Contact: Dr Shafagh Waters or Dr John Widger

> CF-IDEA: Cystic Fibrosis – Insulin Deficiency, Early Action Trial

The CF-IDEA trial (Cystic Fibrosis – Insulin Deficiency, Early Action) aims to determine whether starting insulin treatment before the onset of diabetes (earlier than current practice) will improve the health of children with Cystic Fibrosis by improving body weight and lung function.
Contact: Dr Shafagh Waters or Dr John Widger

> Harnessing the protective functions of stress granules in CF

Stress granules contribute to cell survival and are formed in response to certain cellular stress.  In this study we test if the oxidative stress caused by CF contributes to cell death in epithelial cells, such as those lining the respiratory and gastrointestinal tracts, by inhibiting stress granule formation. Compounds that promote stress granules will also be assessed.
Contact: Dr Shafagh Waters or Dr John Widger